Academic Materials
Simultaneous Measurement of Glucose and HbA1c in Clinical Chemistry Analyzer
The determination of glucose and HbA1c is essential to diagnose diabetes mellitus. HbA1c is a form of hemoglobin chemically linked to glucose. Glucose is in serum or plasma and HbA1c is inside of RBCs. Glucose can be determined directly from serum and plasma.
On the other hand, HbA1c is determined from lysing solution after RBCs are lysed. Clinical chemistry analyzers equipped with lysing function enable hemolysis of RBCs and the simultaneous determination of glucose and HbA1c.
Latex Immunoturbidimetric Assay
Hb and HbA1c can be released from erythrocytes by hemolysis treatment. Latex particles are immobilized with HbA1c in the sample. Immobilized latex particles by Hb and HbA1c react with mouse anti-human HbA1c monoclonal antibody and goat anti-mouse IgG polyclonal antibody. The agglutination of latex, HbA1c and two types of antibodies is proportional to the percentage of HbA1c. Therefore, the percentage of HbA1C can be determined.
Mechanism of Simultaneous Determination of Glucose and HbA1c from Whole Blood Sample in CA-800
Clinical chemistry analyzer, the CA-800, has the function to lyse RBCs on board. RBCs are lysed in cuvette with lysing solution.
This lysed sample is used for HbA1c determination. This enables the simultaneous determination of Glucose and HbA1c from one blood collecting tube with anticoagulant.
NGSP Certificate
The combination between Furuno CA-800, Centronic HbA1c reagent and calibrator gets NGSP certificate of traceability every year.
Correlation
Centronic HbA1c reagent shows good correlation to enzymatic methodology, too.
Reagent Package Information
| Product Name | Product No. | FURUNO Analyzer | Configuration | ||
|---|---|---|---|---|---|
| R1 | R2 | Other | |||
|
|
GF02917084-F3 | CA-270 CA-400 CA-800 |
3 × 67 mL | 3 × 17 mL | - |
| GF02917084-F | 6 × 67 mL | 6 × 17 mL | - | ||
| GF02917084-F4 | 4 × 20 mL | 4 × 6 mL | - | ||
| GF02917084-F8 | 8 × 20 mL | 8 × 6 mL | - | ||
|
|
HF04917040-F | CA-270 CA-800 |
1 × 30 mL | 12 × 20 mL | Lysing solution 2 × 60mL |
|
|
CCALHbA1cT | CA-270 CA-400 CA-800 |
4 levels × 0.5 mL | ||
|
CNORMHbA1c | 1 levels × 0.5 mL | |||
|
CPATHHbA1c | 1 levels × 0.5 mL | |||
Reagents are manufactured by Centronic GmbH certified according to ISO13485.
FURUNO Analyzers
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Clinical Chemistry Analyzer Model CA-800
800 tests/hour throughput capability (maximum 1200 tests/hour with ISE). Fully direct automatic HbA1c measurement without manual sample pretreatment.
Optional rack system available for place and go sample process. -
Clinical Chemistry Analyzer Model CA-400
Mid-range throughput (400 tests/hour, 560 tests/hour with ISE).
Main instrument for midsize workload laboratory or backup unit for high workload laboratory. -
Clinical Chemistry Analyzer Model CA-270
Compact desk-top instrument with 270 tests/hour (maximum 450 tests/hour with ISE).
Functionality of a high throughput instrument in compact desktop form ideal in any type of laboratories.